Some methodological problems in the determination of antioxidant activity using chromogen radicals: a practical case

The two most widely used chromogen compounds to measure the antioxidant activity of biological material are the ABTS(+) and the DPPH radicals. Both present excellent stability in terrain assay conditions but also show several important differences in their response to antioxidants and in their manipulation. In this study, we present the interferences, at different wavelengths, caused by plant-derived materials (citrus juices and wines) using the two above chromogens to measure their antioxidant activity. (C) 2001 Elsevier Science Ltd. All rights reserved.