期刊
JOURNAL OF NEUROIMMUNOLOGY
卷 111, 期 1-2, 页码 102-108出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0165-5728(00)00373-8
关键词
astrocytoma cells; chemotaxis; calcium mobilization; formyl peptide receptors; cytokines
资金
- NCI NIH HHS [N01-CO-56000] Funding Source: Medline
Activation of astrocytes is important in the pathogenesis of a variety of diseases in the central nervous system, such as infection and neurodegeneration. We found that the bacterial chemotactic peptide, N-formyl-methionyl-leucyl-phenylalanine (fMLF) induced potent migration and Ca2+ mobilization in human astrocytoma cell lines. The effect of fMLF was pertussis toxin-sensitive, suggesting the involvement of seven transmembrane, G protein-coupled receptor(s) for fMLF. Scatchard analyses revealed that astrocytoma cell Lines express both high- and low-affinity binding sites for [H-3]fMLF. RT-PCR confirmed the expression of transcripts of fMLF receptors, the high-affinity FPR and the low-affinity FPRL1 by these cells. Both fMLF and F peptide, a synthetic peptide domain of HIV-1 envelope protein which specifically activates FPRL1, increased secretion of IL-6 by astrocytoma cells. Our study demonstrates for the first time that FPR and FPRL1 expressed by astrocytoma cell lines are functional, and suggests a molecular basis for the involvement of these receptors in host defense in the brain. (C) 2000 Elsevier Science B.V. All rights reserved.
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