Adaptive aberration correction in a two-photon microscope

We demonstrate aberration correction in two-photon microscopy. Specimen-induced aberrations were measured with a modal wavefront sensor, implemented using a ferroelectric liquid crystal spatial light modulator (FLCSLM). Wavefront correction was performed using the same FLCSLM. Axial scanned (x-z) images of fluorescently labelled polystyrene beads using an oil immersion lens show restored sectioning ability at a depth of 28 mum in an aqueous specimen.