期刊
JOURNAL OF BIOTECHNOLOGY
卷 196, 期 -, 页码 33-41出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2015.01.006
关键词
Haematococcus pluvialis; Beta carotene ketolase; Astaxanthin; Agrobacterium; Transformation
资金
- DST (Department of Science and Technology), New Delhi
- CSIR-CFTRI (Central Food Technological Research Institute)
- CSIR, Govt. of India
Astaxanthin a high-value ketocarotenoid used in the pharmaceutical and nutraceutical industries is mainly produced from green alga, Haematococcus pluvialis. It is biosynthesized by the action of key enzyme, beta-carotene ketolase (BKT) on beta-carotene through intermediates echinenone and canthaxanthin. In this study, the beta-carotene ketolase (bkt) gene was isolated from H. pluvialis and cloned in a vector pRT100 and further mobilized to a binary vector pCAMBIA 1304. The T-DNA of pCAMBIA 1304, which consists of cloned bkt, was successfully transformed to H. pluvialis through Agrobacterium mediation. The cloning and transformation of bkt in H. pluvialis was confirmed by Southern blotting and also by PCR analysis. Total carotenoids and astaxanthin content in the transformed cells were found to be 2-3-foldhigher, while the intermediates like echinenone and canthaxanthin were found to be 8-10-fold higher than in the control cells. The expression level of carotenogenic genes like phytoene synthase (psy), phytoene desaturase (pds), lycopene cyclase (lcy), bkt, and beta -carotene hydroxylase (bkh) were found to be higher in transformed cells compared to the non-transformed (NT) H. pluvialis. (C) 2015 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据