期刊
JOURNAL OF BACTERIOLOGY
卷 182, 期 22, 页码 6412-6417出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JB.182.22.6412-6417.2000
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资金
- NIGMS NIH HHS [GM47461] Funding Source: Medline
The predominant photolesion in the DNA of UV-irradiated dormant bacterial spores is the thymine dimer 5-thyminyl-5,6-dihydrothymine, commonly referred to as spore photoproduct (SP), A major determinant of SP repair during spore germination is its direct reversal by the enzyme SP base, encoded by the splB gene in Bacillus subtilis. SpIB protein containing an N-terminal tag of six histidine residues [(6His)SpIB] was purified from dormant B, subtilis spores and shown to efficiently cleave SP but not cyclobutane cis,syn thymine-thymine dimers in vitro. In contrast, SpIB protein containing an N-terminal 10-histidine tag [(10His)SpIB] purified fi om an Escherichia coli overexpression system was incompetent to cleave SP unless the 10-His tag was first removed by proteolysis at an engineered factor Xa site. To assay the parameters of binding of SpIB protein to UV-damaged DNA, a 35-bp double-stranded oligonucleotide was constructed which carried a single pair of adjacent thymines on one strand. Irradiation of the oligonucleotide in aqueous solution or at 10% relative humidity resulted in formation of cyclobutane pyrimidine dimers (Py lozenge Py) or Sp, respectively. (10His)SpIB was assayed for oligonucleotide binding using a DNase I protection assay. In the presence of (10His)SpIB, the SP-containing oligonucleotide was selectively protected from DNase I digestion (half-life, >60 min), while the Py lozenge Py-containing oligonucleotide and the unirradiated oligonucleotide were rapidly digested by DNase I (half-lives, 6 and 9 min, respectively). DNase I footprinting of (10His)SpIB bound to the artificial substrate was carried out utilizing the P-32 end-labeled 35-bp oligonucleotide containing SP, DNase I footprinting showed that SpIB protected at least a 9-bp region surrounding SP from digestion with DNase I with the exception of two DNase I-hypersensitive sites within the protected region. (10His)SpIB also caused significant enhancement of DNase I digestion of the SP-containing oligonucleotide for at least a full helical turn 3' to the protected region. The data suggest that binding of SP base to SP causes significant bending or distortion of the DNA helix in the vicinity of the lesion.
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