期刊
JOURNAL OF BIOTECHNOLOGY
卷 194, 期 -, 页码 12-18出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2014.11.032
关键词
Immobilization lipase; Hollow nanospheres; Kinetic resolution; Ketoprofen vinyl ester
资金
- National Program on Key Basic Research Project of China (973 Program) [2013CB328900]
- National Natural Science Foundation of China [21321061, J1310008, J1103315]
The aim of this study was to improve the ability of Aspergillus terreus lipase to separate the racemic ketoprofen vinyl ester into individual enantiomers using hollow self-assembly alginate-graft-poly(ethylene glycol)/alpha-cyclodextrins (Alg-g-PEG/alpha-CD) spheres as enzyme immobilization carriers. The morphology and size of the Alg-g-PEG/alpha-CD particles were investigated by transmission electron microscopy (TEM) and were found to be nanoscale. To facilitate recycling, calcium alginate (CA) beads were developed to encapsulate Alg-g-PEG/alpha-CD particles, thereby producing Alg-g-PEG/alpha-CD/CA composite beads. The influence of buffer pH and enzyme concentration during immobilization was studied along with the biocatalyst's kinetic parameters. When the immobilized enzyme was under optimal conditions in the resolution reaction, maximal conversion (approximately 45.9%) and enantioselectivity (approximately 128.8) were obtained. The immobilized A. terreus lipase maintained excellent performance even after 20 reuses and retained nearly 100% of its original activity after 24 weeks of storage at 4 degrees C. (C) 2014 Elsevier B.V. All rights reserved.
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