期刊
JOURNAL OF BIOTECHNOLOGY
卷 216, 期 -, 页码 131-139出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2015.10.016
关键词
Glutathione enriched yeast; Fed-batch strategy; Scale-up; Glutathione overproduction; Repeated fed-batch; Cysteine incorporation yield
资金
- Federal Ministry of Economic Affairs and Energy via cooperation project of the Central Innovation Program SME (ZIM) [VP2132312 MDO]
In the following work a high cell density fed-batch process with Saccharomyces cerevisiae coupled with a high efficient incorporation of cysteine for glutathione (GSH) overproduction was developed. Therefore, a feeding strategy based on the respiratory quotient (RQ) was applied to ensure high biomass (96.1 gib. Furthermore, the optimal cysteine concentration and time of cysteine addition were investigated. Low concentrations of cysteine at late fermentation phases resulted in relatively high incorporation yields of about 0.40 mol/mol and maintained the physiology of cultivated yeast. By changing the cysteine feeding from standard single shot to continuous addition, an often observed cell specific toxicity, triggered by high cysteine concentrations, could be prevented and the cysteine incorporation yield (0.54 +/- 0.01 mol/mol) and GSH content (1650.7 +/- 42.8 mg/l; 1.76 +/- 0.08%) were maximized, respectively. The developed process was transferred from laboratory into pilot plant scale. Further, the reduced cell specific toxicity enabled the development of a repeated fed-batch procedure with a suitable performance concerning cysteine incorporation yield (0.40 +/- 0.1 mol/mol), biomass (84.2 +/- 1.2 g/l) and GSH content (1304.7 +/- 61.4 mg/l). (C) 2015 Elsevier B.V. All rights reserved.
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