期刊
CARDIOVASCULAR RESEARCH
卷 48, 期 2, 页码 300-309出版社
OXFORD UNIV PRESS
DOI: 10.1016/S0008-6363(00)00180-2
关键词
arrhythmia (mechanisms); heart failure; membrane currents; K-channel
Objective: Heart failure in patients and in animal models is associated with action potential prolongation of the ventricular myocytes. Changes in several membrane currents have been already demonstrated to underlie this prolongation. However, information on the two components (I-Kr and I-Ks) of the delayed rectifier potassium current (I-K) in rapid pacing induced heart failure is lacking. Methods and results: Action potentials and whole-cell currents, I-K, I-tol, I-Kl, and ICa-L were recorded in apical myocytes of left ventricle from 10 rabbits subjected to left ventricular pacing at 350-380 beats/min for 3-4 weeks and 10 controls with shan operation. Action potential duration at 90% repolarization (APD(90)) was prolonged in myocytes from failing hearts compared to controls at both cycle lengths of 333 and 1000 ms. Both E-4031-sensitive and -resistant components of I-K (I-Kr, I-Ks) in myocytes from failing hearts were significantly less than those of control hearts; tail current densities of I-Kr and I-Ks following depolarization to +50 mV were 0.62+/-0-05 vs. 0.96+/-0.12 pA/pF (P<0.05), and 0.27+/-0.08 vs. 0.52+/-0.08 pA/pF (P<0.05), respectively. There was no significant difference between control and failing myocytes in the voltage- and time-dependence of activation of total I-K, I-Kr and I-Ks. The peak of L-type Ca2+ current (ICa-L) was significantly reduced in myocytes from failing hearts (at +10 mV, -9.29+/-0.52 vs. -12.28+/-1.63 pA/pF, P<0.05), as was the Ca2+-independent transient outward current (I-tol; at +40 mV,4.8+/-0.9 vs. 9.6+/-1.3 pA/pF, P<0.05). Steady state I-V curve for I-Kl was similar in myocytes from failing and control hearts. Conclusions: Decrease of I-K (both I-Kr and I-Ks) in addition to reduced I-tol, may underly action potential prolongation at physiological cycle length and thereby contribute to arrhythmogenesis in heart failure. (C) 2000 Elsevier Science B.V. All rights reserved.
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