期刊
ANALYTICA CHIMICA ACTA
卷 899, 期 -, 页码 85-90出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2015.09.051
关键词
Thrombin; Thrombin aptamer; Fluorescence; CdS quantum dots
资金
- National Natural Science Foundation of China [21075050, 21275063]
- Science and Technology Development Project of Jilin Province, China [20110334]
We fabricated a novel fluorescence biosensor for the selective detection of thrombin by using bovine serum albumin-capped CdS quantum dots (BSA-CdS QDs). Two kinds of designed DNA (DNA1 and DNA2) could bind to CdS QDs through the electrostatic interaction between DNA and Cd2+ on the surface of CdS QDs. The obtained DNA/BSA-CdS QDs kept stable in the solution with the fluorescence intensity obviously enhanced. Hairpin structure of DNA1contained two domains, one is the aptamer sequence of thrombin and the other is the complementary sequence of DNA2. When thrombin was added, it would bind to DNA1 and induce the hairpin structure of DNA1 changed into G-quadplex structure. Meanwhile, DNA2 would transfer from the surface of CdS QDs to DNA1 via hybridization, which resulted in the removal of DNA1 and DNA2 from the surface of CdS QDs, and led to the fluorescence intensity of CdS QDs reduced. Thus, the determination of thrombin could be achieved by monitoring the change of the fluorescence intensity of CdS QDs. The present method is simple and fast, and exhibits good selectivity for thrombin over other proteins. We have successfully detected thrombin in human serum samples with satisfactory results. (C) 2015 Elsevier B.V. All rights reserved.
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