期刊
BIOFOULING
卷 28, 期 7, 页码 671-680出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/08927014.2012.701286
关键词
biofilm; CO2 aerosols; Bdellovibrio bacteriovorous; bacterial predation; safety and exposure
资金
- National Research Foundation of Korea (NRF)
- Ministry of Education, Science and Technology [2010-0015377, 2010-0028073]
- UNIST (Ulsan National Institute of Science and Technology)
This study evaluated predation with Bdellovibrio bacteriovorous and CO2 aerosol spraying to remove fluorescent Escherichia coli biofilms from silicon chips. Initial tests found that 7.5 x 10(5) viable E. coli cells were dispersed into the surrounding environment during aerosol treatment. The total number dispersed per test decreased to only 16 for predated biofilms. This is nearly 50,000-fold lower compared to untreated chips and 1000-fold lower compared to chips soaked in HEPES buffer only. Both scanning electron microscopy (SEM) and fluorescent microscopy analyses confirmed that predation alone did not completely eradicate the biofilm population. When used in conjunction with CO2 aerosols, however, no fluorescent signals remained and the SEM pictures showed a pristine surface devoid of bacteria. Consequently, this study demonstrates these two methods can be used with each other to significantly remove biofilms from surfaces while also significantly reducing the likelihood of human exposure to potential pathogens during their removal.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据