Seventy-six Landrace and four Large White x Landrace pigs (n = 80) of 90-134 kg liveweight were randomly allocated to a 2x2x2 factorial experiment to determine the effect of halothane genotype [heterozygous for the halothane gene (Nn) and homozygous dominant (NN)], pre-slaughter handling (minimal and negative) and stunning method (CO2 stunning and electrical) on pork quality. The rate of muscle pH decline post-slaughter of the nz, longissimus thoracis et lumborum (LTL) muscle was faster in Nn pigs compared with NN pigs (0.86 and 0.30 pH units/h, respectively). Pork from Nn pigs was also paler in colour, had higher percentage drip loss and purge and lower sarcoplasmic and myofibrillar protein solubility compared with NN pigs. Pork from CO2 stunned pigs had a lower drip loss compared to pork from electrically stunned pigs (5.80 and 7.28%, respectively - means of both genotypes combined). Tenderness of pork assessed at 24 h post-slaughter was not influenced by genotype, pre-slaughter handling or stunning method. However, pork from Nn pigs aged for 5 days post-slaughter was less tender than NN pigs (5.84 and 4.84 kg, respectively). Pale, soft and exudative pork was produced in all negatively handled Nn pigs, regardless of stunning method. The average amount of ecchymosis-affected muscle trimmed from carcasses of electrically stunned pigs was higher compared to pigs stunned with CO2 (65 and 0.7 g, respectively). These data indicate that although halothane status was the most important factor influencing pork quality, pre-slaughter handling and stunning method also influenced meat and carcass quality. (C) 2000 Elsevier Science Ltd. All rights reserved.
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