期刊
BIOFOULING
卷 27, 期 7, 页码 729-737出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/08927014.2011.600830
关键词
mussel adhesive protein; fp-5; adhesion; coating; bulk-scale
资金
- National Research Laboratory Program [ROA-2007-000-20066-0]
- Ministry of Education, Science and Technology, Korea
Mussel adhesive proteins (MAPs) attach to all types of inorganic and organic surfaces, even in wet environments. MAP of type 5 (fp-5), in particular, has been considered as a key adhesive material. However, the low availability of fp-5 has hampered its biochemical characterization and practical applications. Here, soluble recombinant fp-5 is mass-produced in Escherichia coli. Tyrosinase-modified recombinant fp-5 showed similar to 1.11 MPa adhesive shear strength, which is the first report of a bulk-scale adhesive force measurement for purified recombinant of natural MAP type. Surface coatings were also performed through simple dip-coating of various objects. In addition, complex coacervate using recombinant fp-5 and hyaluronic acid was prepared as an efficient adhesive formulation, which greatly improved the bulk adhesive strength. Collectively, it is expected that this work will enhance basic understanding of mussel adhesion and that recombinant fp-5 can be successfully used as a realistic bulk-scale bioadhesive and an efficient surface coating material.
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