期刊
JOURNAL OF EXPERIMENTAL MEDICINE
卷 192, 期 9, 页码 1365-1372出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1084/jem.192.9.1365
关键词
hematopoiesis; Jagged-1; Notch; stem cells; development
The Notch ligand, Jagged-1, plays an essential role in tissue formation during embryonic development of primitive organisms. However, Little is known regarding the role of Jagged-1 in the regulation of tissue-specific stem cells or its function in humans. Here, we show that uncommitted human hematopoietic cells and cells that comprise the putative blood stem cell microenvironment express Jagged-1 and the Notch receptors. Addition of a soluble form of human Jagged-1 to cultures of purified primitive human blood cells had modest effects in augmenting cytokine-induced proliferation of progenitors. However, intravenous transplantation of cultured cells into immunodefcient mice revealed that human (h)Jagged-l induces the survival and expansion of human stem cells capable of pluripotent repopulating capacity. Our findings demonstrate that hJagged-1 represents a novel growth factor of human stem cells, thereby providing an opportunity for the clinical utility of Notch ligands in the expansion of primitive cells capable of hematopoietic reconstitution.
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