期刊
GENES & DEVELOPMENT
卷 14, 期 22, 页码 2869-2880出版社
COLD SPRING HARBOR LAB PRESS
DOI: 10.1101/gad.849600
关键词
epigenetics; gene silencing; methylation; Cre-lox; site-specific recombination
In an effort to control the variability of transgene expression in plants, we used Cre-lax mediated recombination to insert a gus reporter gene precisely and reproducibly into different target loci. Each integrant line chosen for analysis harbors a single copy of the transgene at the designated target site. At any given target site, nearly half of the insertions give a full spatial pattern of transgene expression. The absolute level of expression, however, showed target site dependency that varied up to 10-fold. This substantiates the view that the chromosome position can affect the level of gene expression. An unexpected finding was that nearly half of the insertions at any given target site failed to give a full spatial pattern of transgene expression. These partial patterns of expression appear to be attributable to gene silencing, as low gus expression correlates with DNA methylation and low transcription. The methylation is specific for the newly integrated DNA. Methylation changes are not found outside of the newly inserted DNA. Both the full and the partial expression states are meiotically heritable. The silencing of the introduced transgenes may be a stochastic event that occurs during transformation.
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