期刊
JOURNAL OF CHROMATOGRAPHY A
卷 898, 期 2, 页码 227-234出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0021-9673(00)00873-6
关键词
mould; building materials; ergosterol
Ergosterol content of building materials was quantified using gas chromatography-tandem mass spectrometry (GC-MS-MS) in an ion trap with external ionisation. Hydrolysing the samples by classic extraction at 85 degreesC for 90 min in vials was faster, more precise and safer than microwave assisted extraction. [4-(2)H(2)]ergosterol was synthesised and used as internal standard, giving method standard deviation of 5-10% from 10 to 30 ng to 10-15 mug ergosterol in the sample. The use of GC-MS-MS meant that no solid-phase extraction clean-up was needed, so one person could easily prepare 40-80 samples per day. (C) 2000 Elsevier Science BN. All rights reserved.
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