期刊
VACCINE
卷 19, 期 7-8, 页码 810-815出版社
ELSEVIER SCI LTD
DOI: 10.1016/S0264-410X(00)00246-2
关键词
DNA vaccines; tetanus; codon usage; murine model
Two related DNA vaccine vector plasmids, harbouring either wild-type (pcDNA3/ntetC) or synthetic codon optimised (pcDNA3/stetC) DNA encoding fragment C (TetC) of tetanus toxin were constructed. COS-7 cells transformed with pcDNA3/ stetC reproducibly expressed higher levels of TetC than similar cells transformed with pcDNA3/ntetC. BALB/c mice immunised intramuscularly with pcDNA3/stetC produced significantly higher levels of anti-TetC antibodies in their serum in the weeks following vaccination compared to mice immunised with pcDNA3/ntetC, even when differences in the CpG content between the two sequences were controlled for using non-expressing DNA. (C) 2000 Elsevier Science Ltd. All rights reserved.
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