期刊
BIOESSAYS
卷 34, 期 4, 页码 320-327出版社
WILEY-BLACKWELL
DOI: 10.1002/bies.201100125
关键词
cellular; correlated; cryo-light; morphology; multi-modal; three-dimensional
资金
- US Department of Energy, Office of Biological and Environmental Research [DE-AC02-05CH11231]
- National Center for Research Resources of the National Institutes of Health [P41RR019664]
- National Institutes of General Medicine of the National Institutes of Health [GM63948]
- National Institutes of Health NRSA [1 T32 GMO66698]
- US Department of Energy, Office of Science
Soft X-ray tomography (SXT) is an imaging technique capable of characterizing and quantifying the structural phenotype of cells. In particular, SXT is used to visualize the internal architecture of fully hydrated, intact eukaryotic and prokaryotic cells at high spatial resolution (50?nm or better). Image contrast in SXT is derived from the biochemical composition of the cell, and obtained without the need to use potentially damaging contrast-enhancing agents, such as heavy metals. The cells are simply cryopreserved prior to imaging, and are therefore imaged in a near-native state. As a complement to structural imaging by SXT, the same specimen can now be imaged by correlated cryo-light microscopy. By combining data from these two modalities specific molecules can be localized directly within the framework of a high-resolution, three-dimensional reconstruction of the cell. This combination of data types allows sophisticated analyses to be carried out on the impact of environmental and/or genetic factors on cell phenotypes.
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