期刊
NEUROSCIENCE RESEARCH
卷 38, 期 4, 页码 325-329出版社
ELSEVIER SCI IRELAND LTD
DOI: 10.1016/S0168-0102(00)00188-7
关键词
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT); lactate dehydrogenase; cytotoxicity assay; astrocyte; neuron; culture
3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay and lactate dehydrogenase (LDH) release assay have been widely used for evaluating cell viability in culture. MTT reduction assay measures the redox activity of living cells, while LDH assay measures the activity of LDH released into the medium from dead cells. In this paper, we introduce a quick and simple method or measuring cellular MTT reduction and LDH release with the same dye, MTT. The substrate mixture for measuring LDH activity contained lactate, B-nicotinamide adenine dinucleotide, 1-methoxyphenazine methosulfate, MTT and Triton X-100. When the medium containing LDH was mixed with the substrates, MTT was converted into MTT formazan in proportion to LDH activity. This method was successfully applied for evaluating t-butyl hydroperoxide toxicity in cultured I at cortical astrocytes and glutamate toxicity in cultured rat hippocampal neurons. Our method is economical and convenient especially for measuring cellular MTT reduction and LDH release in the same culture. (C) 2000 Elsevier Science Ireland Ltd and the Japan Neuroscience Society. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据