期刊
BIOELECTROCHEMISTRY
卷 52, 期 2, 页码 151-159出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/S0302-4598(00)00097-0
关键词
LK sheep; Na/K pump; KCl co-transport; anti-L; phosphorylation
We investigated the role of protein phosphorylation in the action of anti-L on low potassium (LK) sheep red cells. Anti-L stimulated the Na/K pump by four- to fivefold, but Na/K pump activity in anti-L-stimulated or in control cells was unaffected by protein kinase/protein phosphatase (PK/PP) inhibitors. KCI co-transport activity was inhibited by anti-L (about 50%). Go-transport was stimulated by staurosporine; and inhibited by calyculin A, okadaic acid, tyrphostin B46 and genistein; with a similar pattern in both control and anti-L-treated cells. O-2 sensitivity of KCl co-transport was similar in control and anti-L-treated cells. Neither control nor anti-L-stimulated Na/K pump activities were O-2 sensitive. Incubation with urea stimulated KCI co-transport in both control and anti-L-treated cells. Inhibition of co-transport by anti-L was unaffected by low concentrations of urea but was reduced at higher urea concentrations. Na/K pump activity of control cells was unaffected by incubation with urea, but that in cells stimulated by anti-L was reduced, though not significantly. Under high hydrostatic pressure, KCI co-transport was stimulated, and the inhibitory effects of PP inhibition (okadaic acid), anti-L or combinations of the two were reduced. Results suggest that anti-L does not affect K+ transport in LK sheep red cells via protein phosphorylation. (C) 2000 Elsevier Science S.A. All rights reserved.
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