期刊
FEMS MICROBIOLOGY ECOLOGY
卷 34, 期 2, 页码 121-128出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0168-6496(00)00080-5
关键词
soil; denitrifrication; denitrifier; amplified ribosomal DNA restriction analysis; 16S rDNA; phylogenetic diversity
类别
Bacteria capable of denitrification are spread among phylogenetically diverse groups. In the present investigation, molecular methods (amplified ribosomal DNA restriction analysis (ARDRA) and partial 16S rDNA gene sequencing) were used to determine the genetic diversity of culturable denitrifying soil bacteria. The purpose of this work was to study the microbial density and diversity of denitrifying communities isolated from two luvisols and a rendosol. The denitrifying bacterial density was significantly higher in the two luvisols (3 x 10(6) and 4 x 10(6) bacteria g(-1) dry soil) than in the rendosol (4 x 10(5) bacteria g(-1) dry soil). Denitrifying isolates from soils were grouped according to the similarity of their restriction patterns into 26 ARDRA types. Interestingly ARDRA analysis suggests that some denitrifying isolates are specific to a soil type while others seem to be geographically widespread. The number of individual isolates found in each ARDRA type appeared to be highly variable between the two sampling dates but some denitrifying types were capable of persisting in soil. The tree obtained from the partial sequences revealed five major branches exhibiting highest identity to the following genera: (i) Burkholderia-Ralstonia, (ii) Pseudomonas, (iii) Xanthomonas-Frateuria, (iv) Bacillus and (v) Streptomyces. Our 16S rDNA-based analysis clearly reveals broad diversity exceeding that previously described in the literature. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. Ail rights reserved.
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