In this article we review tandem mass spectral approaches to the structural characterization of flavonoid glycosides which are ubiquitous secondary plant metabolites. It is demonstrated how structural information on O-diglycosides can be obtained on (i) the carbohydrate sequence, (ii) the aglycone part, and (iii) the linkage in the diglycosidic part. Both the positive and negative ion modes have been employed.
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