期刊
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
卷 48, 期 12, 页码 1609-1616出版社
SAGE PUBLICATIONS LTD
DOI: 10.1177/002215540004801204
关键词
monoclonal antibody; CD3 epsilon chain; T-cell lymphomas; T-cell receptor assembly; immunohistochemistry; paraffin sections
类别
We generated a new monoclonal antibody (MAb), F7.2.38, by immunizing mice with CD3 epsilon gamma delta /CD3 omega complexes purified from human T-cells by OKT3 MAb-Sepharose affinity chromatography. Immunoprecipitation experiments and Western blotting analysis showed that MAb F7.2.38 recognized the CD3 epsilon chain in CD3 epsilon cDNA-transfected FOX B-cells and in Various T-cell lines. Using flow cytometry on permeabilized or intact cells, the epitope was found to be located in the cytoplasmic tail of the CD3 epsilon chain. Immunohistochemical staining on paraffin-embedded sections showed that the reactivity of MAb F7.2.38 was comparable to that of the commercially available anti-CD3 epsilon polyclonal antibody. Of the 52 well-characterized T-cell lymphomas, 41 were positive for F7.2.38 (79%), whereas all 37 B-cell lymphomas and 69 non-lymphoid tumors were unreactive. This new antiCD3 epsilon antibody would be particularly useful for phenotyping T-cell lymphomas on routinely processed paraffin-embedded tissue sections.
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