期刊
BIOENERGY RESEARCH
卷 6, 期 3, 页码 991-999出版社
SPRINGER
DOI: 10.1007/s12155-013-9332-5
关键词
Butanol tolerance; Jatropha seed cake; Acid pretreatment; Scale up
资金
- Reliance Life Sciences Pvt. Ltd.
n-Butanol fermentation using Clostridium strains suffers from low titers due to the inability of the strains to tolerate n-butanol. The current study demonstrates a process to get high titer of n-butanol in a single batch mode from the renewable feedstock jatropha seed cake by employing Clostridium acetobutylicum. Chemical mutagenesis was done for improvement of the strain for better n-butanol tolerance and production. Optimization of the parameters resulted in 13.2 g L-1 of n-butanol in 120 h using acid-treated jatropha seed cake hydrolysate (7 % w/v) in anaerobic sugar medium. The process was scaled up to 15 L level, yielding 18.6 g L-1 of n-butanol in 72 h. The strain was found to be tolerant up to 30 g L-1 n-butanol under optimized conditions. The n-butanol tolerance was accompanied by over-expression of the stress response protein, GroEL, change in fatty acid profile, and ability to accumulate rhodamine 6G in the strain. The study has a significant impact on economically producing n-butanol from biomass.
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