期刊
BIOENERGY RESEARCH
卷 4, 期 4, 页码 225-245出版社
SPRINGER
DOI: 10.1007/s12155-011-9125-7
关键词
Lignocellulose; Cellulose; Cellulosic ethanol; Pretreatment; Enzymatic hydrolysis; Multi-enzyme complex; Enzyme recycling; High solids; Bioreactors; Metabolic engineering; Pentose fermentation; Tolerance; Substrate characteristics; Cellulase adsorption
资金
- Natural Science Foundation of China [20976130, 20806057]
- International Science and Technology Cooperation Program [2006DA62400]
- National Key Technology RD program [2007BAD42B02]
- Ministry of Education [NCET-08-0386, 200800561004]
- Tianjin RD program [2010-BK17C004]
Biofuels produced from lignocellulosic biomass can significantly reduce the energy dependency on fossil fuels and the resulting effects on environment. In this respect, cellulosic ethanol as an alternative fuel has the potential to become a viable energy source in the near future. Over the past few decades, tremendous effort has been undertaken to make cellulosic ethanol cost competitive with conventional fossil fuels. The pretreatment step is always necessary to deconstruct the recalcitrant structures and to make cellulose more accessible to enzymes. A large number of pretreatment technologies involving physical, chemical, biological, and combined approaches have been developed and tested at the pilot scale. Furthermore, various strategies and methods, including multi-enzyme complex, non-catalytic additives, enzyme recycling, high solids operation, design of novel bioreactors, and strain improvement have also been implemented to improve the efficiency of subsequent enzymatic hydrolysis and fermentation. These technologies provide significant opportunities for lower total cost, thus making large-scale production of cellulosic ethanol possible. Meanwhile, many researchers have focused on the key factors that limit cellulose hydrolysis, and analyzing the reaction mechanisms of cellulase. This review describes the most recent advances on process intensification and mechanism research of pretreatment, enzymatic hydrolysis, and fermentation during the production of cellulosic ethanol.
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