期刊
BIOENERGY RESEARCH
卷 4, 期 3, 页码 193-200出版社
SPRINGER
DOI: 10.1007/s12155-010-9112-4
关键词
Feedstock pretreatment; Enzymatic hydrolysis; Particle size reduction; Acid digestion
资金
- Joint KSU/Specialty Enzymes Project at Grain Science and Industry Department
- Iowa State University Agricultural Experiment Station
- Div Of Industrial Innovation & Partnersh
- Directorate For Engineering [0917984] Funding Source: National Science Foundation
Cellulosic feedstocks for bioenergy differ in composition and processing requirements for efficient conversion to chemicals and fuels. This study discusses and compares the processing requirements for three lignocellulosic feedstocks-soybean hulls, wheat straw, and de-starched wheat bran. They were ground with a hammer mill to investigate how differences in composition and particle size affect the hydrolysis process. Enzyme hydrolysis was conducted using cellulase from Trichoderma reesei at 50A degrees C and pH 5. Ground fractions were also subjected to dilute sulfuric acid treatment at 125A degrees C, 15 psi for 30 min prior to cellulase treatment. Reducing particle size of biomass resulted in segregated components of feedstock. Grinding wheat straw to particle size < 132 mu m resulted in measured lignin content from 20% to a parts per thousand 5% and reduced hemicellulose content. Reducing lignin content increased the effectiveness of enzyme hydrolysis of wheat straw. Particles sized < 132 mu m exhibited the highest soluble sugar release upon hydrolysis for all three feedstocks studied. Hemicellulose digestion improved with dilute sulfuric acid treatment with residual hemicellulose content < 5% in all three feedstocks after acid treatment. This enhanced the cellulase action and resulted in approximately 1.6-fold increase in sugar availability in de-starched wheat bran and a parts per thousand 1.5-fold for wheat straw and soybean hulls. Higher sugar availability in wheat bran after acid-mediated enzyme treatment correlated to higher ethanol yields during yeast fermentation compared with soybean hulls and wheat straw.
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