期刊
BIOELECTROMAGNETICS
卷 35, 期 3, 页码 170-180出版社
WILEY
DOI: 10.1002/bem.21833
关键词
ischemia necrosis of femoral head; pulsed electromagnetic fields simulation; glucocorticoids; peroxisome proliferator-activated receptor-2 (PPAR-2); Runt-related transcription factor 2 (Runx2)
资金
- National Natural Science Foundation of China [81301592]
- Natural Science Foundation of Hubei Province of China [2010CDB06905, 2012FKB04446]
- Fundamental Research Funds for the Central Universities
- Independent Research Project of Wuhan University for Graduate Students [2012302020207]
This study was designed to investigate the effects of pulsed electromagnetic fields (PEMF) on the balance of adipogenesis and osteogenesis on steroid-induced osteonecrosis of the femoral head (OFH) in rats. Forty-two rats were divided into three groups: Steroid group (S, n=16); Steroid+PEMF group (S+P, n=16); and Control group (C, n=10). For groups S and S+P, all rats were first intravenously given 10 mu g/kg lipopolysaccharide on day 1, and then intramuscularly injected with 20mg/kg methylprednisolone acetate on days 2, 3, and 4, with an interval of 24h. After 4 weeks, the S+P group was treated with PEMF (4.5-ms square pulse, repeated at 15Hz, with a peak of 1.2mT) for 4h a day for the next 8 weeks. Group S was not exposed to PEMF. Group C was chosen as the control group, without steroid use and exposure to PEMF. After 8 weeks of treatment, the histological changes, and mRNA and protein expressions of PPAR-2 and Runx2 were measured and analyzed. Compared with the S group, lower incidence of osteonecrosis (31% vs. 69%, P<0.05) and empty osteocyte lacuna rate (36.16 +/- 15.34 vs. 59.55 +/- 21.70, P<0.01) was observed in the S+P group. Furthermore, PEMF suppressed the expressions of PPAR-2 and improved the expressions of Runx2 in the femoral head (P<0.05). All data suggest that PEMF is an effective physiotherapy in the treatment of steroid-induced ONFH, and the possible underlying mechanisms include protecting the balance between adipogenesis and osteogenesis. Bioelectromagnetics. 35:170-180, 2014. (c) 2014 Wiley Periodicals, Inc.
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