期刊
NATURE BIOTECHNOLOGY
卷 18, 期 12, 页码 1287-1292出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/82407
关键词
ribosome display; in vitro selection; single-chain antibody fragments; Human Combinatorial Antibody Library; affinity maturation
Here we applied ribosome display to in vitro selection and evolution of single-chain antibody fragments (scFvs) from a large synthetic library (Human Combinatorial Antibody Library; HuCAL) against bovine insulin. In three independent ribosome display experiments different clusters of closely related scFvs were selected, all of which bound the antigen with high affinity and specificity. All selected scFvs had affinity-matured up to 40-fold compared to their HuCAL progenitors, by accumulating point mutations during the ribosome display cycles. The dissociation constants of the isolated scfvs were as low as 82 pM, which validates the design of the naive library and the power of this evolutionary method. We have thus mimicked the process of antibody generation and affinity maturation with a synthetic library in a cell-free system in just a few days, obtaining molecules with higher affinities than most natural antibodies.
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