4.2 Article

Lung Cancer A549 Cells Migrate Directionally in DC Electric Fields With Polarized and Activated EGFRs

期刊

BIOELECTROMAGNETICS
卷 30, 期 1, 页码 29-35

出版社

WILEY
DOI: 10.1002/bem.20436

关键词

A549 cells; metastasis; epidermal growth factor receptors; direct-current electric fields; galvanotaxis

资金

  1. National Natural Science Foundation of China [30670510]

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Endogenous direct-current electric fields (dcEFs) occur in vivo in the form of epithelial transcellular potentials or neuronal field potentials. A variety of cells respond to dcEFs by migrating directionally, and this is termed galvanotaxis. The mechanism by which dcEFs direct cell movement, however, is not yet understood, and the effects on lung cancer cells are entirely unknown. We demonstrated that cultured human lung adenocarcinoma A549 cells migrate toward the cathode in applied dcEFs at 3 V/cm. Fluorescence microscopy showed that both epidermal growth factor receptors (EGFRs) and F-actin are polarized to the cathode. EGFR inhibitors. cetuximab and AG1478, reduced the ink,ration rate and directed motility in dcEFs. Western blots showed that ERK and AKT signaling pathways were prominently promoted by dcEFs. EGFR inhibitors could reduce this promotion but not completely. These data suggest that polarization of EGFRs and the activation of their downstream signals play an important role in the galvanotaxis of A549 cells in dcEFs. Bioelectromagnetics 30:29-35, 2009. (C) 2008 Wiley-Liss, Inc.

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