期刊
BIOELECTROCHEMISTRY
卷 96, 期 -, 页码 37-42出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bioelechem.2014.01.001
关键词
Glucose biosensor; Microperoxidase-11; Layer-by-layer technique; Liposome; Biosensor
资金
- FAPESP
- CNPq
- CAPES
- nBioNet network (Brazil)
An important step in several bioanalytical applications is the immobilization of biomolecules. Accordingly, this procedure must be carefully chosen to preserve their biological structure and fully explore their properties. For this purpose, we combined the versatility of the layer-by-layer (LbL) method for the immobilization of biomolecules with the protective behavior of liposome-encapsulated systems to fabricate a novel amperometric glucose biosensor. To obtain the biosensing unit, an LbL film of the H2O2 catalyst polypeptide microperoxidase-11 (MP-11) was assembled onto an indium-tin oxide (ITO) electrode followed by the deposition of a liposome-encapsulated glucose oxidase (GOx) layer. The biosensor response toward glucose detection showed a sensitivity of 0.91 +/- 0.09 (mu A/cm(2))/mM and a limit of detection (LOD) of 8.6 +/- 1.1 mu M, demonstrating an improved performance compared to similar biosensors with a single phospholipid-liposome or even containing a non-encapsulated GOx layer. Finally, glucose detection was also performed in a zero-lactose milk sample to demonstrate the potential of the biosensor for food analysis. (C) 2014 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据