期刊
BIOELECTROCHEMISTRY
卷 83, 期 -, 页码 25-30出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bioelechem.2011.08.001
关键词
Biosensor; Heavy metal ions; Inhibitory analysis; Enzyme; Conductometric transducer
资金
- NASU
- STCU [4591]
A differential pair of planar thin-film interdigitated electrodes, deposited on a ceramic pad, was used as a conductometric transducer. The three-enzyme system (invertase, mutarotase, glucose oxidase), immobilized on the transducer surface, was used as a bioselective element. The ratio between enzymes in the membrane was found experimentally considering the highest biosensor sensitivity to substrate (sucrose) and heavy metal ions. Optimal concentration of sucrose for inhibitory analysis was 1.25 mM and incubation time in the investigated solution amounted to 10-20 min. The developed biosensor demonstrated the best sensitivity toward ions Hg2+ and Ag2+. A principal possibility of the biosensor reactivation either by EDTA solution after inhibition with silver ions or by cysteine solution after inhibition with mercury ions was shown. (C) 2011 Elsevier B.V. All rights reserved.
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