期刊
BIOELECTROCHEMISTRY
卷 86, 期 -, 页码 30-37出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bioelechem.2012.01.005
关键词
Electrochemical genosensor; Neisseria gonorrhoeae; Sexually transmitted disease; Nanocomposite; Nucleic acid hybridization
资金
- Council of Scientific and Industrial Research (CSIR), India
- Department of Science & Technology (DST) [DST/TSG/ME/2008/18, GAP-070932]
- CSIR-EMPOWER [OLP-102232]
- Department of Biotechnology, Govt. of India [DBT/GAP070832]
The nanocomposite based on polyaniline (PANI)-iron oxide nanoparticles (nFe(3)O(4)) and multi walled carbon-nanotubes (CNT) has been fabricated onto indium tin oxide (ITO) coated glass plate via facile electrochemical synthesis of polyaniline in presence of nFe(3)O(4) (similar to 20 nm) and CNT (20-80 nm in diameter). The results of transmission electron microscopic studies show evidence of coating of PANI and nFe(3)O(4) onto the CNT. The PANI-nFe(3)O(4)-CNT/ITO nanoelectrode has been characterized by Fourier transform infrared spectroscopy, X-ray diffraction and scanning electron microscopy studies. The biotinylated nucleic acid probe sequence consisting of 20 bases has been immobilized onto PANI-nFe(3)O(4)-CNT/ITO nanoelectrode using biotin-avidin coupling. It is shown that the PANI-nFe(3)O(4)-CNT platform based biosensor can be used to specifically detect bacteria (N. gonorrhoeae) at minute concentration as low as (1 x 10(-19) M) indicating high sensitivity within 45 s of hybridization time at 298 K by differential pulse voltammetry using methylene blue as electroactive indicator. This bacterial sensor has also been tested with 4 positive and 4 negative PCR amplicons of gonorrhoea affected patient samples. The results of these studies have implications towards the fabrication of a handheld device for Neisseria gonorrhoeae detection that may perhaps result in a decrease in the human immunodeficiency virus infections. (C) 2012 Elsevier B.V. All rights reserved.
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