期刊
BIOELECTROCHEMISTRY
卷 79, 期 1, 页码 11-16出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bioelechem.2009.09.013
关键词
Conducting polymers; Polypyrrole; EIS immunosensor; Nanotechnology; NanoBioTechnology
资金
- Agency for International Science and Technology Development Programmes in Lithuania
Polypyrrole (Ppy) has been shown as a matrix for label-free electrochemical immunosensor based on electrochemical impedance spectroscopy (EIS) measurements. The immunosensing system model presented here was based on bovine leukemia virus (BLV) protein (gp51) entrapped within electrochemically-synthesized polypyrrole (Ppy/gp51). This Ppy/gp51 layer interacted with antibodies against gp51 (anti-gp51-Ab) that are present in significant concentration in the blood serum of BLV infected cattle. After this interaction protein complex (Ppy/gp51/anti-gp51-Ab) was formed. The horseradish peroxidase (HRP) labeled secondary antibodies (Ab*) against anti-gp51-Ab were applied as agents interacting with Ppy/gp51/anti-gp51-Ab and forming the large protein complex (Ppy/gp51/anti-gp51-Ab/Ab*). The EIS study was performed for electrodes modified with different Ppy layers described here and an optimal equivalent circuit was adopted for evaluation of EIS spectra, it was a major outcome of this study. (C) 2009 Elsevier B.V. All rights reserved.
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