期刊
BIOELECTROCHEMISTRY
卷 77, 期 1, 页码 64-68出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bioelechem.2009.05.008
关键词
Cellulose; Ionic liquids; Direct electron transfer; Glucose oxidase; Carbon nanotube
资金
- U.K.'s Engineering and Physical Sciences Research Council's Supergen5 Biological Fuel Cells Consortium programme (EPSRC) [EP/D047943/1]
- EPSRC [EP/D047943/1] Funding Source: UKRI
- Engineering and Physical Sciences Research Council [EP/D047943/1] Funding Source: researchfish
Conductive cellulose-multiwalled carbon nanotube (MWCNT) matrix with a porous structure and good biocompatibility has been prepared using a room temperature ionic liquid (1-ethyl-3-methylimidazolium acetate) as solvent. Glucose oxidase (GOx) was encapsulated in this matrix and thereby immobilized on a glassy carbon surface. The direct electron transfer and electrocatalysis of the encapsulated GOx has been investigated using cyclic voltammetry and chronoamperometry. The GOx exhibited a pair of stable, well defined and nearly symmetric reversible redox peaks. The experimental results also demonstrate that the immobilized GOx retains its biocatalytic activity toward the oxidation of glucose and therefore can be employed in a glucose biosensor. The results Show that the bioelectrode modified by the cellulose-MWCNT matrix has potential for use in biosensors and other bioelectronics devices. (C) 2009 Elsevier B.V. All rights reserved.
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