期刊
BIOELECTROCHEMISTRY
卷 74, 期 2, 页码 246-253出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bioelechem.2008.08.007
关键词
Glucose oxidase; Chitosan; NdPO4 nanoparticles; Direct electrochemistry; Biosensor
资金
- National Science Foundation of China [20675062, 20875076]
- Research Fund for the Doctoral Program of Higher Education [20060697013]
The direct electrochemistry Of glucose oxidase (GOx) immobilized on a composite matrix based on chitosan (CHIT) and NdpO(4) nanoparticles (NPs) underlying on glassy carbon electrode (GCE) was achieved. The cyclic voltammetry and electrochemical impedance spectroscopy were used to characterize the modified electrode. in deaerated buffer solutions, the cyclic voltammetry of the composite films of GOx/NdPO4 NPs/CHIT showed a pair of well-behaved redox peaks that are assigned to the redox reaction of GOx, confirming the effective immobilization of GOx on the composite film. The electron transfer rate constant was estimated to be 5.0 s(-1). The linear dynamic range for the detection of glucose was 0.15-10 mM with a correlation coefficient of 0.999 and the detection limit was estimated at about 0.08 rnM (S/N=3). The calculated apparent Michaelis-Menten constant was 2.5 mM, which suggested a high affinity of the enzyme-substrate. The immobilized GOx in the NdPO4 NPs/CHIT composite film retained its bioactivity. Furthermore. the method presented here can be easily extended to immobilize and obtain the direct electrochemistry of other redox enzymes or proteins. (C) 2008 Elsevier B.V. All rights reserved.
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