期刊
ANIMAL GENETICS
卷 31, 期 6, 页码 396-399出版社
BLACKWELL SCIENCE LTD
DOI: 10.1046/j.1365-2052.2000.00684.x
关键词
bovine myostatin gene; double-muscling phenotype; multiplex oligonucleotide ligation assay
We herein describe a procedure that allows for simultaneous genotyping of six loss-of-function mutations in the bovine myostatin gene associated with the double-muscling phenotype. The proposed method relies on a multiplex oligonucleotide ligation assay and detection of the fluorescently labelled products using automatic sequencers.
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