期刊
BIOELECTROCHEMISTRY
卷 74, 期 1, 页码 83-89出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bioelechem.2008.04.019
关键词
Hydrogenases; Biocatalysis; Electrochemistry; Activation
资金
- Fundacao para a Ciencia e Tecnologia, Portugal [SFRH/BD/2917/2000]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/2917/2000] Funding Source: FCT
Direct electrochemical response was first time observed for the redox centers of Desulfovibrio gigas [NiFe]-Hase, in non-turnover conditions, by cyclic voltammetry, in solution at glassy carbon electrode. The activation of the enzyme was achieved by reduction with H-2 and by electrochemical control and electrocatalytic activity was observed. The inactivation of the [NiFe]-Hase was also attained through potential control. All electrochemical data was obtained in the absence of enzyme inhibitors. The results are discussed in the context of the proposed mechanism currently accepted for activation/inactivation of [NiFe]-Hases. (C) 2008 Elsevier B.V. All rights reserved.
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