期刊
FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY
卷 368, 期 8, 页码 836-838出版社
SPRINGER-VERLAG
DOI: 10.1007/s002160000595
关键词
-
A fluorimetric method for the determination of vitamin B-12 has been developed. The fluorescence emission was measured at lambda (ex)/lambda (em)275/305 nm in phosphate buffer solution (pH 7.0), and the experimental variables and possible interference were studied. The linear calibration range was 1.000 ng/mL to 100.0 ng/mL with a correlation coefficient of 0.9994 and a detection limit of 0.1 ng/mL. The method is rapid, simple and highly sensitive. It was used to determine vitamin B12 in pharmaceutical preparations. The recovery was 96%-98% and the relative standard deviation was in the range of 1.8%-2.7%. The results agreed with those obtained by spectrophotometry.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据