期刊
JOURNAL OF BACTERIOLOGY
卷 182, 期 24, 页码 6958-6963出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JB.182.24.6958-6963.2000
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- FIC NIH HHS [TW00976] Funding Source: Medline
- NIAAA NIH HHS [AA11393] Funding Source: Medline
- NIAID NIH HHS [R01 AI049174, AI49174, R21 AI049174] Funding Source: Medline
Mycothiol is a novel thiol produced only by actinomycetes and is the major low-molecular-weight thiol in mycobacteria, Mycothiol was previously shown to be synthesized from 1-D-myo-inosityl-2-amino-2-deoxy-alpha -D-glucopyranoside by ligation with cysteine followed by acetylation, A novel mycothiol-dependent detoxification enzyme, mycothiol conjugate amidase, was recently identified in Mycobacterium smegmatis and shown to have a homolog, Rv1082, in Mycobacterium tuberculosis, In the present study we found that a protein encoded by the M, tuberculosis open reading frame Rv1170, a homolog of Rv1082, possesses weak mycothiol conjugate amidase activity but shows substantial deacetylation activity with 1-D-myo-inosityl-2-acetamido-2-deoxy-alpha -D-glucopyranoside (GlcNAc-Ins), a hypothetical mycothiol biosynthetic precursor. The availability of this protein enabled us to develop an assay for GlcNAc-Ins, which was used to demonstrate that GlcNAc-Ins is present in M, smegmatis at a level about twice that of mycothiol, It was shown that GlcNAc-Ins is absent in mycothiol-deficient mutant strain 49 of M, smegmatis and that this strain can concentrate GlcNAc-Ins from the medium and convert it to mycothiol, This demonstrates that GlcNAc-Ins is a key intermediate in the pathway of mycothiol biosynthesis, Assignment of Rv1170 as the gene coding the deacetylase in the M, tuberculosis genome represents the first identification of a gene of the mycothiol biosynthesis pathway. The presence of a large cellular pool of substrate for this enzyme suggests that it may be important in regulating mycothiol biosynthesis.
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