期刊
PEPTIDES
卷 21, 期 12, 页码 1865-1870出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/S0196-9781(00)00332-6
关键词
rat; pig; spleen; intestine; mRNA-protein mismatch
资金
- NIDA NIH HHS [T32 DA-07239, DA-10200] Funding Source: Medline
- NINDS NIH HHS [NS-17702] Funding Source: Medline
This study was undertaken to examine the presence of functional nociceptin/orphanin FQ (N/OFQ) receptors in the immune system. Receptor mRNA signals were detected by RT-PCR in porcine thymus, lymph nodes, spleen and freshly-isolated splenocytes; the distribution of prepro-nociceptin/-orphanin FQ (PP-N/-OFQ) mRNA was similar, with the exception of lymph nodes However, specific [H-3]nociceptin binding sites were not detected in rat or porcine lymphoid tissues, and 0.1-100 nM nociceptin had no effect on forskolin-stimulated cyclic AMP concentrations in porcine splenocytes. Thus, it appears that nociceptin/orphanin FQ receptor mRNA, but not a functional receptor protein is expressed in the immune system. (C) 2000 Elsevier Science Inc. All rights reserved.
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