期刊
BIODEGRADATION
卷 20, 期 6, 页码 813-826出版社
SPRINGER
DOI: 10.1007/s10532-009-9269-8
关键词
Bacterial consortium; Biodegradation; Azo dye; Cr(VI); Real-time PCR; FTIR
资金
- Department of Biotechnology, Ministry of Science and Technology, India
- Australian Government Department of Education Science and Training (DEST)
Bacterial consortium-AIE2 with a capability of contemporaneous Cr(VI) reduction and azo dye RV5 decolourization was developed from industrial wastewaters by enrichment culture technique. The 16S rRNA gene based molecular analyses revealed that the consortium bacterial community structure consisted of four bacterial strains namely, Alcaligenes sp. DMA, Bacillus sp. DMB, Stenotrophomonas sp. DMS and Enterococcus sp. DME. Cumulative mechanism of Cr(VI) reduction by the consortium was determined using in vitro Cr(VI) reduction assays. Similarly, the complete degradation of Reactive Violet 5 (RV5) dye was confirmed by FTIR spectroscopic analysis. Consortium-AIE2 exhibited simultaneous bioremediation efficiencies of (97.8 +/- A 1.4) % and (74.1 +/- A 1.2) % in treatment of both 50 mg l(-1) Cr(VI) and RV5 dye concentrations within 48 h of incubation at pH 7 and 37A degrees C in batch systems. Continuous bioreactor systems achieved simultaneous bioremediation efficiencies of (98.4 +/- A 1.5) % and (97.5 +/- A 1.4) % after the onset of steady-state at 50 mg l(-1) input Cr(VI) and 25 mg l(-1) input RV5 concentrations, respectively, at medium dilution rate (D) of 0.014 h(-1). The 16S rRNA gene copy numbers in the continuous bioreactor as determined by real-time PCR assay indicated that Alcaligenes sp. DMA and Bacillus sp. DMB dominated consortium bacterial community during the active continuous bioremediation process.
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