期刊
BIOCONTROL SCIENCE AND TECHNOLOGY
卷 22, 期 11, 页码 1362-1372出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09583157.2012.729027
关键词
Esteya vermicola; formulation; stress resistance; pinewood nematode; virulence
资金
- Ministry of Agriculture and Forestry, South Korea
The endoparasitic nematophagous fungus, Esteya vermicola, has potential as a biocontrol agent against pinewood nematode, Bursaphelenchus xylophilus. An E. vermicola conidial formulation was developed to improve conidial resistance to ultraviolet (UV), drought and heat stress. The effective concentration of each protective additive [UV protectant [fulvic acid (FA) and skim milk (SM)]; drought protectant (sorbitol) and heat protectant (calcium chloride)] was determined based on the germination rate of E. vermicola conidia after exposure to the different stressors. A combination of 0.2% FA and 4% SM, 5% sorbitol and 0.05% calcium chloride provided the most effective protection. In addition, the concentrations of spreader-sticker and antibiotic were also decided. The final formulation could be used to improve the resistance of E. vermicola conidia to multiple stressors and to increase nematode mortality compared with unformulated conidia.
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