期刊
HUMAN REPRODUCTION
卷 15, 期 12, 页码 2460-2463出版社
OXFORD UNIV PRESS
DOI: 10.1093/humrep/15.12.2460
关键词
cryopreservation; nitrogen vapour; safety; sperm banks
A recent consultation documentation by the Human Fertilisation and Embryology Authority (HFEA) which focused on the safe cryopreservation of gametes and embryos highlighted the need for a review of the way that fertility clinics in the UK store potentially infective material, The main points for consideration mere to: (i) ensure containers used for cryopreservation are guaranteed by manufacturers to withstand low temperatures; (ii) use secondary containers, i.e. 'double bagging' of samples if stared in the Liquid phase; and (iii) store in nitrogen vapour as a 'safer' alternative. In this article we examine a number of issues related to vapour storage which need careful consideration, including safety, cost and the effectiveness of various storage techniques in maintaining gamete and embryo viability, We also discuss the effectiveness of vapour storage in comparison with current liquid nitrogen storage techniques. In conclusion we propose that fertility clinics should be compelled to review their cryopreservation procedures, not just because of new legislation or indeed fear of litigation but by a moral obligation.
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