期刊
CURRENT OPINION IN BIOTECHNOLOGY
卷 11, 期 6, 页码 527-531出版社
ELSEVIER SCI LTD
DOI: 10.1016/S0958-1669(00)00140-3
关键词
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The enzyme transketolase has been employed as a catalyst for asymmetric carbon-carbon bond formation in the synthesis of biologically important molecules. A number of important parameters have been addressed including substrate specificity, over-expression of the protein in suitable host systems, scale-up of the reaction and use of transketolase in multi-enzyme experiments. X-ray structural studies have been used to probe the origin of the asymmetry of the carbon-carbon bond-forming process.
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