期刊
ANALYTICAL CHEMISTRY
卷 72, 期 23, 页码 5774-5778出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac0006371
关键词
-
资金
- NCRR NIH HHS [R01 RR14173-02] Funding Source: Medline
Glass microchips, integrating chemical derivatization reactions, electrophoretic separations, and amperometric detection, have been developed. The performance of the new integrated microfabricated devices is demonstrated for rapid on-chip measurements of amino acids utilizing precolumn reactions of amino acids with o-phthaldialdehyde/2-mercaptoethanol to generate electroactive derivatives that are separated electrophoretically and detected at the end-column electrochemical detector. The influence of the sample/reagent mixing ratio, reagent concentrations, driving voltage, detection potential, and other variables is explored. The integrated microsystem offers a rapid (6 min) simultaneous measurements of eight amino acids, down to similar to2.5 x 10(-6) M (5 fmol) level, with linearity up to the 2 x 10(-4) M level examined, and good reproducibility (RSD = 2.2-2.756), A step of the driving voltage is used for decreasing the migration time of late-eluting components and reducing the overall analysis time. The integrated microfabricated device expands the scope of on-chip electrochemical detection to nonelectro-active analytes and holds promise of being a powerful analytical tool.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据