期刊
EMBO JOURNAL
卷 19, 期 23, 页码 6408-6418出版社
OXFORD UNIV PRESS
DOI: 10.1093/emboj/19.23.6408
关键词
general secretion pathway; pilus retraction; secretin; twitching motility; type IV pili
资金
- NIAID NIH HHS [AI27837] Funding Source: Medline
- NIGMS NIH HHS [2 T32 GM07315] Funding Source: Medline
Type IV pili (Tfp) are a unique class of multifunctional surface organelles in Gram-negative bacteria, which play important roles in prokaryotic cell biology. Although components of the Tfp biogenesis machinery have been characterized, it is not clear how they function or interact. Using Neisseria gonorrhoeae as a model system, we report here that organelle biogenesis can be resolved into two discrete steps: fiber formation and translocation of the fiber to the cell surface. This conclusion is based on the capturing of an intermediate state in which the organelle is retained within the cell owing to the simultaneous absence of the secretin family member and biogenesis component PilQ and the twitching motility/pilus retraction protein PilT. This finding is the first demonstration of a specific translocation defect associated with loss of secretin function, and additionally confirms the role of PilT as a conditional antagonist of stable pilus fiber formation. These findings have important implications for Tfp structure and function and are pertinent to other membrane translocation systems that utilize a highly related set of components.
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