期刊
FEMS MICROBIOLOGY ECOLOGY
卷 34, 期 2, 页码 147-155出版社
OXFORD UNIV PRESS
DOI: 10.1111/j.1574-6941.2000.tb00764.x
关键词
digoxigenin-polymerase chain reaction screening; sulfate-reducing bacterium; ulcerative colitis; colonoscopy
类别
A PCR-based approach combined with microbiological cultivation methods was employed to determine the occurrence of sulfate-reducing bacteria (SRB) in colon biopsy samples from ulcerative colitis patients and from non-colitic controls. The detection of mucosa-associated SRB was carried out by digoxigenin-dUTP-labelled PCR amplification, in liquid Postgate medium B and in a new liquid medium, termed VM medium I. Using Postgate medium B; the growth of SRB was confirmed in 92% of the colitic specimens and in 52% of non-colitic samples. However, PCR analysis and incubation in VM medium I detected SRB in 100% of biopsy material indicating ubiquitous presence of SRB in human colon mucosa. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
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