期刊
BIOCONJUGATE CHEMISTRY
卷 24, 期 10, 页码 1721-1732出版社
AMER CHEMICAL SOC
DOI: 10.1021/bc4002757
关键词
-
类别
资金
- EU through Centre of Excellence of Chemical Biology [SF0180027s08, SF0180019s11, ETF9438]
- EU through Centre of Excellence of Chemical Biology, project HCV-Tech [3.2.0701.11-0015]
- Swedish Research Council (VRNT)
- Cepep AB
In the current work we characterize the uptake mechanism of two NickFect family members, NF51 and NF1, related to the biological activity of transfected plasmid DNA (pDNA). Both vectors condense pDNA into small negatively charged nanoparticles that transfect He La cells with equally high efficacy and the delivery is mediated by SCARA3 and SCARA.5 receptors. NF1 condenses DNA into less homogeneous and less stable nanoparticles than NF51. NF51/pDNA nanoparticles enter the cells via macropinocytosis, while NF1/pDNA complexes use clathrin- or caveolae-mediated endocytosis and macropinocytosis. Analysis of separated endosomal compartments uncovered lysomotropic properties of NF51 that was also proven by cotransfection with chloroquine. In summary we characterize how radical modifications in peptides, such as introducing a kink in the structure of NF51 or including extra negative charge by phospho-tyrosine substitution in NF1, resulted in equally high efficacy for gene delivery, although this efficacy is achieved by using differential transfection pathways.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据