期刊
BIOCONJUGATE CHEMISTRY
卷 23, 期 11, 页码 2159-2167出版社
AMER CHEMICAL SOC
DOI: 10.1021/bc3001968
关键词
-
类别
资金
- USC Molecular Imaging Center
- Department of Energy [DE-SC0002353]
- American Cancer Society [121991-MRSG-12-034-01-CCE]
Although various activatable optical probes have been developed to visualize metalloproteinase (MMP). activities in vivo, precise quantification of the enzyme activity is limited due to the inherent scattering and attenuation (limited depth penetration) properties of optical imaging. In this investigation, a novel activatable peptide probe Cu-64-BBQ650-PLGVR-K(Cy5.5)-E-K(DOTA)-OH was constructed to detect tumor MMP activity in vivo. This agent is optically quenched in its native form, but releases strong fluorescence upon cleavage by selected enzymes. MMP specificity was confirmed both in vitro and in vivo by fluorescent imaging studies. The use of a single modality to image biomarkers/ processes may lead to erroneous interpretation of imaging data. The introduction of a quantitative imaging modality, such as PET, would make it feasible to correct the enzyme activity determined from optical imaging. In this proof of principle report, we demonstrated the feasibility of correcting the activatable optical imaging data through the PET signal. This approach provides an attractive new strategy for accurate imaging of MMP activity, which may also be applied for other protease imaging.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据