Amperometric biosensor for the quantification of gentisic acid using polyphenol oxidase modified carbon paste electrode

The affinity of mushroom polyphenol oxidase (PPO) towards gentisic acid (GA), a metabolite of acetyl salicylic acid (ASA), is demonstrated by spectrophotometry and by electrochemical techniques. The enzyme can selectively recognize GA even in the presence of large excess of ASA and its metabolic derivatives (salicylic acid (SA) and salicyluric acid (SUA)). At -0.150 V, the sensitivity is (6.1 +/- 0.1)x 10(4) NAM(-1), the response is linear up to 2.0 x 10(-4) M and the detection limit is 5.0 x 10(-5) M. The kinetic parameters, obtained from Eadie-Hofstee plots, are I-max = 51.4 nA and K-m(app) = 6.7 x 10(-4) M. (C) 2000 Elsevier Science B.V. All rights reserved.