期刊
PLANT SCIENCE
卷 160, 期 1, 页码 37-47出版社
ELSEVIER SCI IRELAND LTD
DOI: 10.1016/S0168-9452(00)00355-1
关键词
Lotus japonicus; Fabaceae; cytochrome P450; isoflavonoid; vestitol
Treatment of the seedlings of Lotus japonicus, a model legume for molecular genetic studies, with reduced glutathione (GSH) resulted in the accumulation of an isoflavan phytoalexin, vestitol. Using PCR strategies based on the conserved amino acid sequences, full length P450 cDNAs were obtained from GSH-treated seedling roots. When the clones, LjCYP-1 (CYP93C family) (IFS) and isoflavone 2'-hydroxylase (12'H) activities, respectively. The transcription levels of LjCYP-1, LjCYP-2 and isoflavone reductase, which are all involved in vestitol biosynthesis, coordinately increased upon elicitation. Genomic Southern blot analysis indicated that the IFS gene forms a small gene Family and a single copy of the 12'H gene is present in the L. japonicus genome. Molecular biological aspects of P450s involved in the isoflavonoid pathway and the genomic approach to flavonoid metabolism in this unique plant are discussed. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.
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